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For larger proteins, the 20 kd MWCO fiber should be used. It should be used for proteins in the range of 20 kd on up to about 140 kd. For the 5 kd MWCO fiber we define the useful retention as 20 kd. This depends upon the size, shape, post-translational modifications and Stokes radius of the protein, not just the molecular weight. We also speak of the “useful retention” meaning the size of protein that will be retained by 90% or more. The larger cartridges should only be used when significant scale up is desired.Īt FiberCell Systems, we define our molecular weight cut offs at the 50% retention mark as a convention. The medium sized C2011 with a surface area of 3,000 cm2 and a volume of only 20 mL has been shown to out-produce a 10 liter bag type bioreactor by a significant degree. They have specific applications for in vitro toxicology.Īs a general rule the cartridge used will depend on the size and the amount of protein you wish to collect…įiberCell Systems hollow fiber bioreactors are powerful cell culture tools and their small size can be misleading. The cellulosic fibers are generally not intended for use in cell culture, as their low gross filtration rate is not optimal for cell growth. The FiberCell Systems hollow fiber bioreactors are the first bioreactors that are optimized for both adherent and suspension cells. One unique feature of the FiberCell Systems hollow fiber bioreactor is that the fibers are cast with little “waves”, resulting in an even distribution of the fiber bundle within the cartridge shell. Any cell type that will attach to plastic or grow in suspension culture should grow well in the polysulfone cartridge. These characteristics allow for better cell growth and higher fidelity of protein production not seen in cellulosic fibers. Both the 5 kD MWCO and 20 kD MWCO fibers have ten times the gross filtration rate of the equivalent cellulosic fiber for rapid nutrient and waste exchange.
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The hydrophilic polysulfone fiber is specifically selected for excellent cell culture performance. We also offer three different molecular weight cut-offs (MWCO): 5 kD, 20 kD, and. FiberCell Systems offers three types of fibers: polysulfone, cellulosic, and PS+ fibers. A cartridge should be selected based on the intended application.